FIGURE

Fig. 4

ID

ZDB-FIG-140528-8

Publication

Fischer et al., 2013 - Abcb4 acts as multixenobiotic transporter and active barrier against chemical uptake in zebrafish (Danio rerio) embryos

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Fig. 4

Quantification of rhodamine B (RhB) dye accumulation in zebrafish embryos. (A) Amounts of RhB accumulated in 1 hpf, 6 hpf, 12 hpf, 24 hpf and 48 hpf zebrafish embryos that, simultaneously to RhB, were exposed to various concentrations of transporter inhibitors cyclosporin A (CsA), PSC833 or MK571. (B) Amounts of RhB accumulated in 48 hpf zebrafish embryos upon morpholino knock-down of Abcb4 (Abcb4-SP-MO/Abcb4-ATG-MO) or Abcb5 (Abcb5-SP-MO). Controls contained dye only (ctrl, ctrl-MO), the morpholino control (ctrl-MO) served to exclude unspecific morpholino effects on dye accumulation in the embryos. Experiments with CsA (5 µM CsA, ctrl-MO + 5 µM CsA) were set up as comparisons for the effects of this pharmacologic transporter inhibitor on RhB accumulation in the embryos. Values are means +/ standard deviations from three independent experiments. Statistically significant differences from controls were determined with one-way analysis of variance (ANOVA), followed by Dunnett?s test and are indicated by asterisks (P <0.05).

Expression Data

Gene:	abcb4
Fish:	WT
Anatomical Terms:	intestinal bulb intestine
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-abcb4 WT + MO2-abcb4
Conditions:	chemical treatment: cyclosporin A chemical treatment: MK 571
Knockdown Reagents:	MO1-abcb4 MO2-abcb4
Observed In:	efflux transmembrane transporter activity
Stage Range:	4-cell to Long-pec

Phenotype Detail

Acknowledgments

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