ZFIN ID: ZDB-FIG-140527-8
Steiner et al., 2014 - Dynamic gene expression by putative hair-cell progenitors during regeneration in the zebrafish lateral line. Proceedings of the National Academy of Sciences of the United States of America   111:E1393-401 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 4

Fig. 1

Expression of fluorescent proteins in mantle cells of the posterior lateral-line system. (A) Confocal mosaic images of a living alpl:mCherry;Et20 larva at 4 days postfertilization (dpf) demonstrate expression of mCherry (magenta) overlapping with that of GFP (green) in neuromasts and interneuromast cells. (Scale bar: 500 μm.) The same color code applies in CE. (B) A neuromast comprises at least three cell types: hair cells (green), supporting cells (aqua), and mantle cells (magenta). (C) mCherry expression in alpl:mCherry larvae is limited to a subset of cells at the periphery of the neuromast. The image represents a confocal slice through a living alpl:mCherry; Tg(8.0cldnb:lynEGFP)zf106 larva, in which all cells of the neuromast express membrane-tethered GFP. (D) An alpl:mCherry;pou4f3:GFP animal expresses mCherry in peripheral cells, but that marker is excluded from hair cells that express membrane-tethered GFP instead. (E) mCherry and GFP have extensively overlapping but not identical expression patterns in mantle cells of alpl:mCherry;Et20 larvae. The arrowheads indicate two mCh+, GFP cells. (Scale bar: CE, 10 μm.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP ru1011Tg; sqet20Et standard conditions Day 4 interneuromast cell IFL
Day 4 neuromast mantle cell IFL
Day 4 posterior lateral line neuromast IFL
ru1011Tg; zf106Tg standard conditions Day 4 neuromast plasma membrane IFL
GFP ru1011Tg; s356tTg standard conditions Day 4 neuromast hair cell IFL
mCherry ru1011Tg; s356tTg standard conditions Day 4 Not Detected neuromast hair cell IFL
Day 4 neuromast mantle cell IFL
ru1011Tg; sqet20Et standard conditions Day 4 fin IFL
Day 4 interneuromast cell IFL
Day 4 neuromast mantle cell IFL
Day 4 pigment cell IFL
Day 4 posterior lateral line neuromast IFL
ru1011Tg; zf106Tg standard conditions Day 4 neuromast mantle cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA