FIGURE

Fig. S1

ID

ZDB-FIG-140515-22

Publication

van Impel et al., 2014 - Divergence of zebrafish and mouse lymphatic cell fate specification pathways

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Fig. S1

Expression pattern of flt4BAC:mCitrine reporter line at different stages of vascular development.

(A-H) Double transgenic embryos for flt4:mCit (green) and kdrl:mCherry-Caax (red) at different stages of vascular development. (A, B) Initially, the flt4 reporter shows expression in both, arterial and venous ECs with an enriched signal within the venous compartment (26 hpf). (C, D) From about 26 hpf onwards, arterial expression of the construct decreases, so that emerging secondary sprouts can be easily followed at around 36 hpf (arrows). (E, F) At 2 dpf, the flt4 reporter expression is strongly confined to venous derived structures (venous ISV marked by arrowhead) and the signal gradually increases in the lymphatic lineage (see PLs highlighted by arrows). (G, H) By day 5, flt4:mCit expression is still evident in the PCV and venous ISVs (arrow). In addition, lymphatic structures including the TD, ISLVs as well as the DLAV are clearly highlighted throughout the trunk. [DA-dorsal aorta, PCV-posterior cardinal vein, TD-thoracic duct, ISV-intersegmantal vessel, ISLV-intersegmental lymphatic vessel, DLAV-dorsal longitudinal anastomotic vessel, DLLV-dorsal longitudinal lymphatic vessel]

Expression Data

Genes:	Citrine mCherry
Fish:	hu7135Tg; s916Tg
Anatomical Terms:	artery endothelial cell dorsal aorta dorsal longitudinal anastomotic vessel dorsal longitudinal lymphatic vessel endothelial cell intersegmental lymph vessel intersegmental vein intersegmental vessel lymphatic system posterior cardinal vein thoracic duct vascular lymphangioblast vasculature vein endothelial cell
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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