FIGURE

Fig. 1

ID

ZDB-FIG-140507-25

Publication

Follo et al., 2013 - Knock-down of Cathepsin D in zebrafish fertilized eggs determines congenital myopathy

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Fig. 1

CD-knockdown zebrafish morphants exhibit a bent phenotype

(A) Fertilized eggs at the stage of one or two cells were injected with CD–MPO or control MPO (Co). A pool of (not de-yolked) larvae at 3 and 4 dpf was collected and analysed by Western blot for CD expression. The Western blot of three different pools of larvae from three different experiments is shown. In control-injected larvae, the 41 kDa mature CD was detected, whereas in CD–MPO-injected larvae CD was absent. Tubulin and actin were used as reference of protein loading in the lanes. (B) Control and CD–MPO morphants at 1–4 dpf were imaged under the stereo microscope. A representative selection from three different experiments is shown. The bent phenotype is clearly evident in CD–MPO morphants.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ctsd
Knockdown Reagent:	MO1-ctsd
Observed In:	whole organism
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

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