Fig. S5
- ID
- ZDB-FIG-140422-20
- Publication
- Roberts et al., 2014 - Targeted transgene integration overcomes variability of position effects in zebrafish
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PhiC31-mediated transgenic founders show highly reproducible expression patterns. Neuronal expression patterns obtained in integrase injected founders uobL6 IF1, uobL6 IF2, uobL6 IF8 and uobL12 IF1. Red lens indicates legitimate PhiC31-mediated integration into the Xla.crygc:attP-GFP cassette from either Tg(Xla.crygc:attP-GFP)uobL6 or Tg(Xla.crygc:attP-GFP)uobL12. Auto-fluorescent yolk syncytial layer (ysl) is marked by arrows in mCherry images. YFP channel shows EL161-driven expression patterns are listed in Figure 3B. Larvae are at protruding mouth stage (3 dpf ) with anterior to the left. Scale bar indicates 100 μm. |