Fig. 4

The effects of the test compounds on tumor inhibition in LSC-xenografted zebrafish.

(A) Typical images of xenotransplanted zebrafish either not treated or treated with the control drug (imatinib) or an LSC inhibitor (parthenolide). Scale bar: 1.0 mm. (B) Tumor proliferation in xenotransplanted zebrafish. The test compounds (0.5 μM) were administered at 72?96 hpf. The ratio of the fluorescence intensity at 120 and 72 hpf was calculated as an indicator of the increase in tumor size, i.e. tumor proliferation (n = 15?25). P<0.01 vs. no drug; #P<0.05 and ##P<0.01 vs. ALDH- cells. (C) Representative image of the main tumor, angiogenesis, and cell migration. Scale bar: 1.0 mm. (D) Quantitative analysis of cell migration. The number of migrated tumors and their sizes (area) were measured within concentric rings at a defined distance from the main tumor and grouped accordingly. The LSC inhibitors decreased the size and the number of migrated tumors in LSC xenotransplants (n = 15?25). P<0.05 P<0.01 vs. no drug.