FIGURE

Fig. 8

ID

ZDB-FIG-140409-30

Publication

Cruz et al., 2013 - Glucocorticoid receptor, but not mineralocorticoid receptor, mediates cortisol regulation of epidermal ionocyte development and ion transport in zebrafish (danio rerio)

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Fig. 8

Expression patterns of GR mRNA and protein in the ionocytes of adult zebrafish gills.

Representative images of gill paraffin sections labeled with anti-glucocorticoid receptor (GR) (A), anti-alpha sub-unit of N⁺-K⁺-ATPase (NKA) (a marker for NaRCs) (B), and anti-GR and anti-NKA (C). Asterisks indicate gr-expressing cells without an NKA signal (B?C). Gill cryosections reveal co-localization of gr mRNA with NKA (D?E) and anti-H⁺-ATPase (HA) (F?G). After gr mRNA in situ hybridization, nuclear/cellular structure can be visualized with DAPI signals (H?J). Arrows and arrow-head indicate cells with colocalized signals (D?G). Scale bars: 25 μm (A?C) and 5 μm (D?I).

Expression Data

Gene:	nr3c1
Antibodies:	Ab1-atp1a Ab1-nr3c1
Fish:	WT
Anatomical Term:	gill ionocyte
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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