FIGURE

Fig. 6

ID

ZDB-FIG-140313-39

Publication

Fekany et al., 1999 - The zebrafish bozozok locus encodes Dharma, a homeodomain protein essential for induction of gastrula organizer and dorsoanterior embryonic structures

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Fig. 6

Analysis of expression and rescuing potential of dharma RNA in boz mutants. (A,B) Whole-mount in situ hybridization reveals expression of dharma in the dorsal YSL of wild-type embryos at 50% epiboly (A). dharma was not expressed in sibling boz embryos (B). (C,D) Expanded ntl and hgg1 expression in embryos from boz^m168/+ parents injected with dharma at the 1- to 4-cell stage. (E,F) Embryos coinjected with dharma and fluorescent dextran into one cell at the 16-cell stage. Nomarski (E) and fluorescent (F) images reveal that dextran is localized to the blastoderm. (G,H) After injections into the yolk at 1000-cell stage, fluorescent dextran remains confined to the YSL as seen in a Nomarski (G) and fluorescent (H) image. (A,B,EH) Lateral views with animal pole to the top, (E-H) dorsal to the right; (C) animal view. Scale bar, 200 μm.

Expression Data

Gene:	dharma
Fish:	WT dharma^m168/m168 (AB)
Anatomical Term:	yolk syncytial layer dorsal region
Stage:	50%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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