FIGURE

Fig. 3

ID

ZDB-FIG-140305-12

Publication

Yoon et al., 1997 - Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell-stage embryos and is expressed in the primordial germ cells

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Fig. 3

Whole-mount in situ hybridization on early cleavage to domestage embryos. Embryos were hybridized with a vas cDNA fragment riboprobe. (See also Fig. 6 for schematic summary.) All panels show top views of embryos, except in B (side view). vas transcript is detected along the cleavage planes (arrows) of embryos at the 2-cell stage (A,B, same embryo) and 4-cell stage (C). These lines of expression persist through the 8-cell stage (D). vas RNA expression condenses into 4 subcellular clumps by the 32-cell stage (not shown), and remains in this configuration through the 1000-cell stage (E). By the 4000-cell stage (dome stage), vas RNA is no longer subcellularly localized and appears to fill the cytoplasm (F). There are 4-12 vasexpressing cells per embryo at the dome stage. Scale bar, 100 μm.

Expression Data

Gene:	ddx4
Fish:	WT
Anatomical Terms:	blastodisc blastomere germ line cell primordial germ cell
Stage Range:	2-cell to Dome

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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