ZFIN ID: ZDB-FIG-140304-11
Xu et al., 2014 - Swap70b is required for convergent and extension cell movement during zebrafish gastrulation linking Wnt11 signalling and RhoA effector function. Developmental Biology   386(1):191-203 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 4-cell to Protruding-mouth

Fig. 1

Swap70b expression during zebrafish development. (A) Schematic representation of zebrafish Swap70b with putative Ca2+-binding EF-hand motifs at the N-terminus followed by a Def6/Swap70 homology (DSH) domain, a pleckstrin-homology (PH) domain at the centre, and a Dbl-homology-like (DHL) domain that contains a coiled coil motif at the C-terminus. (B) The numbers refer to amino acid identities and similarities between the different orthologues. (C) Expression profiles of swap70/def6 paralogues during zebrafish development. RT-PCR was performed to indicate the expression of five swap70/def6 paralogues at various developmental stages. hpf: hours post-fertilisation. PCR reactions without reverse transcription (RT-) are shown for 24 hpf only and ef1α expression served as an internal control. (D–J) In situ hybridisation with a swap70b-specific antisense probe at different developmental stages: (D) 8-cell; (E) 30% epiboly (30%); (F) 70% epiboly (70%); (G) bud; (H) 5 somites (5 s); (I) 14 somites (14 s); (J) primordium 16 (prim-16) and (K) high pectoral (high pec). Sense control probes did not give rise to any signal (data not shown).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
def6a WT standard conditions 4-cell to Protruding-mouth whole organism RTPCR
def6b WT standard conditions Protruding-mouth whole organism RTPCR
def6c WT standard conditions 1k-cell whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
swap70a WT standard conditions Prim-5 to Protruding-mouth whole organism RTPCR
swap70b WT standard conditions 8-cell whole organism ISH
4-cell to Protruding-mouth whole organism RTPCR
30%-epiboly whole organism ISH
Shield whole organism ISH
Bud optic primordium ISH
5-9 somites optic vesicle ISH
14-19 somites optic vesicle ISH
14-19 somites otic vesicle ISH
14-19 somites ventral mesoderm ISH
Prim-15 caudal vein plexus ISH
Prim-15 otic vesicle ISH
High-pec pectoral fin ISH
High-pec pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 386(1), Xu, X., Shuen, W.H., Chen, C., Goudevenou, K., Jones, P., and Sablitzky, F., Swap70b is required for convergent and extension cell movement during zebrafish gastrulation linking Wnt11 signalling and RhoA effector function, 191-203, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.