|ZFIN ID: ZDB-FIG-140304-11|
|Stage Range:||4-cell to Protruding-mouth|
Swap70b expression during zebrafish development. (A) Schematic representation of zebrafish Swap70b with putative Ca2+-binding EF-hand motifs at the N-terminus followed by a Def6/Swap70 homology (DSH) domain, a pleckstrin-homology (PH) domain at the centre, and a Dbl-homology-like (DHL) domain that contains a coiled coil motif at the C-terminus. (B) The numbers refer to amino acid identities and similarities between the different orthologues. (C) Expression profiles of swap70/def6 paralogues during zebrafish development. RT-PCR was performed to indicate the expression of five swap70/def6 paralogues at various developmental stages. hpf: hours post-fertilisation. PCR reactions without reverse transcription (RT-) are shown for 24 hpf only and ef1α expression served as an internal control. (D–J) In situ hybridisation with a swap70b-specific antisense probe at different developmental stages: (D) 8-cell; (E) 30% epiboly (30%); (F) 70% epiboly (70%); (G) bud; (H) 5 somites (5 s); (I) 14 somites (14 s); (J) primordium 16 (prim-16) and (K) high pectoral (high pec). Sense control probes did not give rise to any signal (data not shown).
|def6a||WT||standard conditions||4-cell to Protruding-mouth||whole organism||RTPCR|
|def6b||WT||standard conditions||Protruding-mouth||whole organism||RTPCR|
|def6c||WT||standard conditions||1k-cell||whole organism||RTPCR|
|swap70a||WT||standard conditions||Prim-5 to Protruding-mouth||whole organism||RTPCR|
|swap70b||WT||standard conditions||8-cell||whole organism||ISH|
|4-cell to Protruding-mouth||whole organism||RTPCR|
|5-9 somites||optic vesicle||ISH|
|14-19 somites||optic vesicle||ISH|
|14-19 somites||otic vesicle||ISH|
|14-19 somites||ventral mesoderm||ISH|
|Prim-15||caudal vein plexus||ISH|
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article.
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Reprinted from Developmental Biology, 386(1), Xu, X., Shuen, W.H., Chen, C., Goudevenou, K., Jones, P., and Sablitzky, F., Swap70b is required for convergent and extension cell movement during zebrafish gastrulation linking Wnt11 signalling and RhoA effector function, 191-203, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.