FIGURE

Fig. 2

ID

ZDB-FIG-140228-2

Publication

Muller et al., 1996 - Expression domains of a zebrafish homologue of the Drosophila pair-rule gene hairy correspond to primordia of alternating somites

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Fig. 2

Cells within the epibolic margin and the tail bud have not been allocated to somites. (A) Fluorescence micrograph of an injection in the hypoblast (arrow) at 80% epiboly. (B-D; 14-somite stage) show examples of the labelling resulting from injections as in A. These embryos were processed for in situ hybridization with digoxigenin-labelled RNA probes followed by anti-fluorescein antibody staining. (B) Fluorescein-labelled cells (brown, arrows) are located in the 9th somite; (D) fluorescein-labelled cells within expression domain VIII. (C) An embryo, in which hypoblastic and epiblastic cells were injected. The former have produced progeny distributed throughout somites 6- 8 (two arrows), the latter contributed to the neural tube, in the presumptive territory of the 19th segment (one arrow). (E) Labelled cells (antifluorescein staining) in somites 15-19, following an injection in the tail bud at 100% epiboly. B and C, same magnification. Scale bars: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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