FIGURE

Fig. 1

ID

ZDB-FIG-140214-49

Publication

Gibbs et al., 2014 - Defective tubulation associated with the myopathy causing S619L DNM2 mutation

Other Figures

Fig. 1

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Fig. 1

T-tubule and sarcoplasmic reticulum abnormalities in DNM2-S619L larval muscle. (A-D) Electron micrographs of longitudinal sections through zebrafish muscle at 3 dpf. (A,C) Muscle from DNM2-WT larvae shows the typical sarcomere striations of vertebrate striated muscle. (B,D) Muscle from DNM2-S619L larvae demonstrates extensive swelling and vacuolization in the region of the SR and T-tubules. Scale bars: 1 �m. (E-H) Confocal micrographs of isolated myofibers subjected to immunofluorescence analysis. (E) Wild-type (WT) myofiber showing the expected pattern of RyR1 staining. (F,G) RyR1 expression is irregular in DNM2-S619L myofibers, and is often found aggregated. (H) α-actinin staining was normal, indicating that other elements of the muscle structure in S619L myofibers are not disturbed.

Expression Data

Antibody:	Ab1-actn
Fish:	AB
Anatomical Term:	skeletal muscle Z disc
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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