ZFIN Image: Gibbs et al., 2014, Fig. 1

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Figure Caption

Fig. 1

T-tubule and sarcoplasmic reticulum abnormalities in DNM2-S619L larval muscle. (A-D) Electron micrographs of longitudinal sections through zebrafish muscle at 3 dpf. (A,C) Muscle from DNM2-WT larvae shows the typical sarcomere striations of vertebrate striated muscle. (B,D) Muscle from DNM2-S619L larvae demonstrates extensive swelling and vacuolization in the region of the SR and T-tubules. Scale bars: 1 �m. (E-H) Confocal micrographs of isolated myofibers subjected to immunofluorescence analysis. (E) Wild-type (WT) myofiber showing the expected pattern of RyR1 staining. (F,G) RyR1 expression is irregular in DNM2-S619L myofibers, and is often found aggregated. (H) α-actinin staining was normal, indicating that other elements of the muscle structure in S619L myofibers are not disturbed.

Figure Data

Expression / Labeling details

Acknowledgments

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