ZFIN ID: ZDB-FIG-140130-14
von Niederhäusern et al., 2013 - Phylogeny and expression of canonical transient receptor potential (TRPC) genes in developing zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   242(12):1427-41 Full text @ Dev. Dyn.
Anatomical Terms:
Stage Range: Prim-5 to Day 5

Fig. 8

A-P: Whole-mount in situ hybridization of zebrafish trpc7a (A-H) and trpc7b (J-P). A-D: trpc7a-expressing olfactory sensory neurons (OSNs) in embryos shown laterally (A,C) and dorsally (B,D). Asterisks mark expression domains in the midbrain. The focal plane in D′ differs from D. E,F: Frontal (E) and ventral (F) views on 3 days postfertilization (dpf) whole-mount larvae, F′ shows a different focus to F. Asterisk in F′ labels midbrain cell clusters expressing trpc7a transiently. G,H: Dorsal (G) and frontal (H) views on larvae 5 dpf. J,K: Dorsal (J) and lateral (K) view of the telencephalic cell clusters expressing trpc7b in embryos 24 hr postfertilization (hpf). L,M: Expression of trpc7b as detected in zebrafish 48 hpf shown in dorsal (L) and lateral (M) views. Focus in L′ is more dorsal compared with L. N,O: Expression in 3 dpf larvae, shown in dorsal (N) and lateral (O, O′) views. P: Dorsal whole-mount view on trpc7b expression in larvae 5 dpf. Die, diencephalon; dTel, dorsal telencephalon; Hb, hindbrain; Mn, motoneurons; RSN, reticulospinal neurons. Scale bar = 100 µm in A (applies to all images without scale bar), L′, P.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
trpc7a WIK standard conditions Prim-5 olfactory receptor cell ISH
Long-pec midbrain ISH
Long-pec olfactory receptor cell ISH
Protruding-mouth midbrain ISH
Protruding-mouth olfactory receptor cell ISH
Day 5 olfactory receptor cell ISH
trpc7b WIK standard conditions Prim-5 brain ISH
Prim-5 diencephalic nucleus ISH
Prim-5 dorsal telencephalon ISH
Prim-5 neuron ISH
Long-pec brain ISH
Long-pec MiD2cm ISH
Long-pec MiD3cm ISH
Long-pec motor neuron ISH
Protruding-mouth brain ISH
Protruding-mouth MiD2cm ISH
Protruding-mouth MiD3cm ISH
Protruding-mouth motor neuron ISH
Day 5 hindbrain nucleus ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.