FIGURE

Fig. 1

ID

ZDB-FIG-140122-30

Publication

Cordero-Maldonado et al., 2013 - Optimization and pharmacological validation of a leukocyte migration assay in zebrafish larvae for the rapid in vivo bioactivity analysis of anti-inflammatory secondary metabolites

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Fig. 1

LPS-enhanced leukocyte migration assay in 4 dpf zebrafish larvae.

All larvae (nacre) are four days post-fertilization (4 dpf), with anterior to the left, scale bar = 10 μm. A, tail of alive larva without tail cut; B, tail of alive larva with tail cut; C?D, whole-mount MPO staining in uncut tails of zebrafish larvae; C, without lipopolysaccharides (LPS) and D, with lipopolysaccharides (+LPS); E?F, whole-mount MPO staining in cut tails of zebrafish larvae; E, without the inclusion of LPS; F, with the inclusion of LPS. Dark-spots (marked by arrows) represent the migrating leukocytes, which are semi-quantified in the region to the right of the dashed red arc.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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