FIGURE

Fig. 4

ID

ZDB-FIG-140114-11

Publication

Charvet et al., 2013 - Knockdown of col22a1 gene in zebrafish induces a muscular dystrophy by disruption of the myotendinous junction

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Fig. 4

TEM reveals defects in MTJ and myosepta ultrastucture, and identifies the rupture site at the outer surface of the basement membrane. Micrographs of 5 dpf uninjected (A,C,E) and MO22-injected (B,D,F-H) larvae. (A,B) Sarcomeric organization of skeletal muscle in morphants (B) and uninjected larvae (A). (C,D) Detail of the sarcolemma and basement membrane structure (arrows) in wild type (C) and morphants (D). (E-H) Detail of MTJ and myoseptum structure in wild type (E) and morphants (F-H). Asterisks show a gap corresponding to detached and retracted fiber (F) and tears between myoseptum and muscle fibers (G,H). At rupture sites, basement membrane remains attached to sarcolemma (G,H, arrows) and is torn away with fiber detachment and retraction (F, arrow). Contrary to control (E), fibroblasts (fb) are observed in damaged myosepta (F-H). ms, myosepta; mf, muscle fiber.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO2-col22a1
Knockdown Reagent:	MO2-col22a1
Observed In:	muscle attachment myotome muscle tendon junction vertical myoseptum fibroblast
Stage:	Day 5

Phenotype Detail

Acknowledgments

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