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ZFIN ID: ZDB-FIG-140107-53
Borovina et al., 2013 - IFT88 Plays a Cilia- and PCP-Independent Role in Controlling Oriented Cell Divisions during Vertebrate Embryonic Development. Cell Reports   5(1):37-43 Full text @ Cell Rep.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: 90%-epiboly to Protruding-mouth

Fig. 1 MZift88 Mutants Do Not Form Cilia

(A and B) Lateral view of a 3-day-old (A) Mift88 embryo and (B) MZift88 mutant embryo exhibiting a curved body axis, pericardial edema (arrowhead), hydrocephalus (asterisk), and a cystic kidney (arrow).

(C and D) Confocal images of (C) WT and (D) MZift88 mesoderm cells, at 9 hpf, expressing Arl13b-GFP (green) and memb-mRFP (red) revealed the presence of cilia on WT cells (asterisks, C). Only small puncta of Arl13b-GFP were visible on MZift88 mutant cells (asterisks, D).

(E) Transmission electron microscopy micrograph of basal body docking in the neural tube of a 30hpf MZift88 mutant embryo.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ift88tz288/tz288 standard conditions 90%-epiboly mesoderm cilium aplastic, abnormal
Prim-15 neural tube lacks all parts of type neural tube axoneme, abnormal
Protruding-mouth brain hydrocephalic, abnormal
Protruding-mouth pericardium edematous, abnormal
Protruding-mouth pronephros cystic, abnormal
Protruding-mouth whole organism curved, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.