|ZFIN ID: ZDB-FIG-140107-53|
|Stage Range:||90%-epiboly to Protruding-mouth|
Fig. 1 MZift88 Mutants Do Not Form Cilia
(A and B) Lateral view of a 3-day-old (A) Mift88 embryo and (B) MZift88 mutant embryo exhibiting a curved body axis, pericardial edema (arrowhead), hydrocephalus (asterisk), and a cystic kidney (arrow).
(C and D) Confocal images of (C) WT and (D) MZift88 mesoderm cells, at 9 hpf, expressing Arl13b-GFP (green) and memb-mRFP (red) revealed the presence of cilia on WT cells (asterisks, C). Only small puncta of Arl13b-GFP were visible on MZift88 mutant cells (asterisks, D).
(E) Transmission electron microscopy micrograph of basal body docking in the neural tube of a 30hpf MZift88 mutant embryo.
|ift88tz288/tz288||standard conditions||90%-epiboly||mesoderm cilium aplastic, abnormal|
|Prim-15||neural tube lacks all parts of type neural tube axoneme, abnormal|
|Protruding-mouth||brain hydrocephalic, abnormal|
|Protruding-mouth||pericardium edematous, abnormal|
|Protruding-mouth||pronephros cystic, abnormal|
|Protruding-mouth||whole organism curved, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.|