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ZFIN ID: ZDB-FIG-140107-53
Borovina et al., 2013 - IFT88 Plays a Cilia- and PCP-Independent Role in Controlling Oriented Cell Divisions during Vertebrate Embryonic Development. Cell Reports   5(1):37-43 Full text @ Cell Rep.
Observed In:
Stage Range: 90%-epiboly to Protruding-mouth

Fig. 1 MZift88 Mutants Do Not Form Cilia

(A and B) Lateral view of a 3-day-old (A) Mift88 embryo and (B) MZift88 mutant embryo exhibiting a curved body axis, pericardial edema (arrowhead), hydrocephalus (asterisk), and a cystic kidney (arrow).

(C and D) Confocal images of (C) WT and (D) MZift88 mesoderm cells, at 9 hpf, expressing Arl13b-GFP (green) and memb-mRFP (red) revealed the presence of cilia on WT cells (asterisks, C). Only small puncta of Arl13b-GFP were visible on MZift88 mutant cells (asterisks, D).

(E) Transmission electron microscopy micrograph of basal body docking in the neural tube of a 30hpf MZift88 mutant embryo.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ift88tz288/tz288 standard conditions 90%-epiboly mesoderm cilium aplastic, abnormal
Prim-15 neural tube lacks all parts of type neural tube axoneme, abnormal
Protruding-mouth brain hydrocephalic, abnormal
Protruding-mouth pericardium edematous, abnormal
Protruding-mouth pronephros cystic, abnormal
Protruding-mouth whole organism curved, abnormal
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.