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Fig. S6

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ZDB-FIG-131218-16

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Sittaramane et al., 2013 - The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function

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Fig. S6

Presence of vangl2 mutant floor plate cells in r4 can block the migration of wildtype FBM neurons.
(A,C) Dorsal views of the hindbrain of wildtype Tg(isl1:GFP) embryos with donor-derived vangl2 mutant (tri-/-) cells in the floor plate (red, arrows). Presence of mutant cells in the host floor plate can block the migration of wildtype FBM neurons out of r4 (black arrowheads). Many FBM neurons (white arrowhead) migrate normally. (B,D) Cross-sections of the embryos at the r4 level (broken lines in A,C) show the presence of donor-derived floor plate cells (red, arrows) and nonmigrated host FBM neurons (arrowheads). The yellow signals in the merged images are largely debris associated with transplantation or artifacts of superimposition of signals in different slices following stacking of confocal images. We verified that ntl morphant cells do not differentiate into FBM neurons (see Materials and Methods). oto, otic vesicle; fp, floor plate.

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Reprinted from Developmental Biology, 382(2), Sittaramane, V., Pan, X., Glasco, D.M., Huang, P., Gurung, S., Bock, A., Li, S., Wang, H., Kawakami, K., Matise, M.P., and Chandrasekhar, A., The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function, 400-412, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.