ZFIN ID: ZDB-FIG-131213-22
Patra et al., 2013 - Organ-specific function of adhesion G protein-coupled receptor GPR126 is domain-dependent. Proceedings of the National Academy of Sciences of the United States of America   110(42):16898-16903 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Pec-fin to Protruding-mouth

Fig. 3 Gpr126 knockdown leads to cardiac abnormalities in zebrafish. (A) Lateral view of a 48-hpf zebrafish embryo after whole-mount in situ hybridization showing gpr126 expression in heart (red arrow) and pericardium (black arrowhead). (B) Parasagittal section confirming cardiac expression of gpr126. (C) Schematic representation of zebrafish full-length Gpr126 (drGpr126) and its NTF part up to the st49 mutation site (drGpr126-NTFΔGPS). (D and E) Confocal sections of hearts from control- and morpholino-injected Tg(myl7:EGFP-HsHRAS)s883 embryos at 80 hpf. In full-length Gpr126-depleted animals (MO1-3) but not in Gpr126-CTF–depleted (MO4) morphants, trabeculation (yellow arrowheads) is perturbed. (Scale bar: 20 μm.) (F) Transmission electron micrographs at 60 hpf reveal that morphants contain elongated Mi with more branched cristae and electron dense precipitates (arrows) compared with WT siblings. (G) Western blot analysis of lysates and conditioned medium from cells overexpressing C-terminal GFP-tagged drGpr126-NTFΔGPS or GFP. Note that both GFP-tagged NTFΔGPS (predicted band size: 113 kDa) and GFP (predicted band size: 27 kDa) were detected in conditioned medium. However, secretion of GFP-tagged NTFΔGPS but not GFP was inhibited by BFA (a blocker of classical trans-Golgi secretory pathway). A, atrium; V, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
adgrg6 AB standard conditions Long-pec atrium ISH
Long-pec cardiac ventricle ISH
Long-pec pericardium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s883Tg + MO1-adgrg6 standard conditions Protruding-mouth heart trabecula formation process quality, normal
s883Tg + MO2-adgrg6 standard conditions Protruding-mouth heart lacks all parts of type trabecular layer, abnormal
Protruding-mouth heart trabecula formation arrested, abnormal
s883Tg + MO4-adgrg6 standard conditions Pec-fin mitochondrion organization process quality, abnormal
Pec-fin myocardium mitochondrial crista increased branchiness, abnormal
Pec-fin myocardium mitochondrion elongated, abnormal
Protruding-mouth heart lacks all parts of type trabecular layer, abnormal
Protruding-mouth heart trabecula formation arrested, abnormal
s883Tg + MO5-adgrg6 standard conditions Protruding-mouth heart lacks all parts of type trabecular layer, abnormal
Protruding-mouth heart trabecula formation arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA