FIGURE

Fig. 5

ID

ZDB-FIG-131108-32

Publication

Anchelin et al., 2013 - Premature aging in telomerase-deficient zebrafish

Other Figures

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Fig. 5

Telomere dysfunction and chromosomal aberrations in G2tert mutant zebrafish. (A) Representative image of the three groups of larvae resulting from tert^-/- × tert^-/- and tert^+/+ × tert^-/- parental matings. The phenotypes were scored as wild type (I), mildly affected (II) and severely affected (III), as indicated in the Materials and Methods. (B) Percentage of larval survival of the three groups observed in A at 1 dpf. (C) Metaphase chromosomes from tert^+/+ and tert^-/- cells were stained with DAPI, and the telomeres were visualized using FISH analysis. The phenotypes of mutant larvae were scored as indicated above. (D) Mean telomeric fluorescence values of 24-hpf embryos (ne100) from wild-type and mutant genetic backgrounds. Data are mean values ± s.e.m. Different letters (a, b, c) denote statistically significant differences among telomeric fluorescence values of each sample according to a Tukey test (P<0.05). (E) Examples of chromosomal aberrations. (F) Frequency of chromosomal aberrations in cell from 24-hour larvae. a.u.f., arbitrary units of fluorescence.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tert^{hu3430/hu3430} tert^hu3430/+
Observed In:	caudal fin pericardial cavity programmed DNA elimination by chromosome breakage whole organism whole organism chromosome, telomeric region
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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