ZFIN Image: Anchelin et al., 2013, Fig. 5

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Fig. 5

Telomere dysfunction and chromosomal aberrations in G2tert mutant zebrafish. (A) Representative image of the three groups of larvae resulting from tert^-/- × tert^-/- and tert^+/+ × tert^-/- parental matings. The phenotypes were scored as wild type (I), mildly affected (II) and severely affected (III), as indicated in the Materials and Methods. (B) Percentage of larval survival of the three groups observed in A at 1 dpf. (C) Metaphase chromosomes from tert^+/+ and tert^-/- cells were stained with DAPI, and the telomeres were visualized using FISH analysis. The phenotypes of mutant larvae were scored as indicated above. (D) Mean telomeric fluorescence values of 24-hpf embryos (ne100) from wild-type and mutant genetic backgrounds. Data are mean values ± s.e.m. Different letters (a, b, c) denote statistically significant differences among telomeric fluorescence values of each sample according to a Tukey test (P<0.05). (E) Examples of chromosomal aberrations. (F) Frequency of chromosomal aberrations in cell from 24-hour larvae. a.u.f., arbitrary units of fluorescence.

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Acknowledgments

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