ZFIN ID: ZDB-FIG-131105-38
Lleras-Forero et al., 2013 - Neuropeptides: developmental signals in placode progenitor formation. Developmental Cell   26(2):195-203 Full text @ Dev. Cell
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Bud to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 4

SST and Noc Control aPPs in Zebrafish

(A–C) Expression of Pax6b (A), pnocb (B), and SST1 (C) in zebrafish at early somite stages: dorsal views, anterior to the left.

(D and E) Pax6b reduction by CSST (E; arrowhead), but not by DMSO (D; white arrowhead).

(F–H) Embryos were injected with control (F) or pnocb MOs (G and H); the latter show Pax6b reduction (arrowheads in G and H). Dotted lines indicate CNS expression of Pax6b.

(I–N) pnocb ATG (I–K) or control (L–N) MO-injected embryos were incubated in DMSO (I and L) or Noc peptide (J, K, M, and N). Dotted lines indicate CNS Pax6b expression.

(O–Q) Embryos were injected with control (O) or pnocb MOs (P and Q). At 24 hpf (frontal views), Pitx3 expression reveals asymmetric, small (P; arrowhead) or almost absent lenses (Q; arrowhead).

(R) Graph shows Pax6b reduction after SST inhibition, SST and Noc inhibition, in Noc morphants and Noc morphants + Noc peptide. Numbers for each treatment are at the top. Brown bars indicate embryos with phenotype, blue bars show normal embryos.

(S) Quantification of pnocb morphants with Pitx3 reduction (yellow) or loss (brown) at early somite stages or 24 hpf.

(T) Model for neuropeptide function.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax6b WT control Bud to 1-4 somites neurogenic field ISH
Bud to 1-4 somites preplacodal ectoderm ISH
WT standard conditions Bud to 1-4 somites neurogenic field ISH
Bud to 1-4 somites preplacodal ectoderm ISH
WT + MO1-pnocb standard conditions Bud to 1-4 somites neurogenic field ISH
Bud to 1-4 somites preplacodal ectoderm ISH
WT + MO1-pnocb + MO2-pnocb standard conditions Bud to 1-4 somites neurogenic field ISH
Bud to 1-4 somites preplacodal ectoderm ISH
WT + MO2-pnocb standard conditions Bud to 1-4 somites neurogenic field ISH
Bud to 1-4 somites preplacodal ectoderm ISH
pitx3 WT control Prim-5 neurogenic field ISH
Prim-5 neurogenic placode ISH
Prim-5 solid lens vesicle ISH
WT + MO1-pnocb standard conditions Prim-5 neurogenic field ISH
Prim-5 neurogenic placode ISH
Prim-5 solid lens vesicle ISH
WT + MO1-pnocb + MO2-pnocb standard conditions Prim-5 neurogenic field ISH
Prim-5 neurogenic placode ISH
Prim-5 solid lens vesicle ISH
WT + MO2-pnocb standard conditions Prim-5 neurogenic field ISH
Prim-5 neurogenic placode ISH
Prim-5 solid lens vesicle ISH
pnocb WT standard conditions Bud to 1-4 somites neurogenic field ISH
Bud to 1-4 somites preplacodal ectoderm ISH
sst1.1 WT standard conditions Bud to 1-4 somites hypoblast ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-pnocb standard conditions Prim-5 solid lens vesicle decreased size, abnormal
WT + MO1-pnocb + MO2-pnocb standard conditions Prim-5 solid lens vesicle decreased size, abnormal
WT + MO2-pnocb standard conditions Prim-5 solid lens vesicle decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 26(2), Lleras-Forero, L., Tambalo, M., Christophorou, N., Chambers, D., Houart, C., and Streit, A., Neuropeptides: developmental signals in placode progenitor formation, 195-203, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell