Fig. 3

Change of miR-3906 level affected the amount of homer-1b mRNA in zebrafish embryos.

(A, D) Compared with WT, injection of miR-3906-MO increased the expression level of homer-1b mRNA in embryos at 24 hpf (A vs. B) and 32 hpf (D vs. E). Injection of miR-3906 dsR (mature double-strand miR-3906) reduced the expression level of homer-1b mRNA in the front of trunk muscle and was absent in the tail region at 24 hpf (A vs. C) and 32-hpf (D vs. F). (G) The expression levels of homer-1b in WT embryos, miR-3906-MO-injected embryos and miR-3906 dsR-injected embryos at 24 hpf and 32 hpf were quantified. Each one was carried out with 100 embryos, and triplicate experiments were performed (n = 3). The numbers shown in the lower-right corner of panels A-F indicate the number of phenotypes out of the total number of embryos examined. SigmaPlot software was used to perform Student’s t-test. *: indicates the difference at P<0.05 level; **: indicates the difference at P<0.01 level.