FIGURE

Fig. 6

ID

ZDB-FIG-131024-4

Publication

Abramsson et al., 2013 - The zebrafish amyloid precursor protein-b is required for motor neuron guidance and synapse formation

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Fig. 6

Impaired patterning of the secondary motor neurons. The Tg(isl1:GFP ) is expressed in SMNs of controls (A) but is reduced in appbMO injected embryos (B). Staining with the SMN-specific antibody zn5 shows irregular projections and stalled outgrowth of the SMN in appb morpholino injected embryos (B) compared to controls (A). The Tg(gata2:GFP) transgene shows that the irregular patterning of extending axons persist in morphants (D) while controls (C) have a regular pattern of SMN by 3 dpf. The dorsolateral fasciculus (DLF) which extends to the end of the spinal cord in controls (E) is projecting out to the tip of the tail fin in appb morphants (arrow, F). The contour of the tail fin is outlined by a dotted line. Bar: 20 μm.

Expression Data

Gene:	GFP
Antibody:	zn-5
Fish:	rw0Tg (AB) zf35Tg (AB) rw0Tg + MO5-appb (AB) zf35Tg + MO5-appb (AB)
Knockdown Reagent:	MO5-appb
Anatomical Term:	secondary motor neuron
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rw0Tg + MO5-appb (AB)
Knockdown Reagent:	MO5-appb
Observed In:	secondary motor neuron axon extension
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 381(2), Abramsson, A., Kettunen, P., Banote, R.K., Lott, E., Li, M., Arner, A., and Zetterberg, H., The zebrafish amyloid precursor protein-b is required for motor neuron guidance and synapse formation, 377-88, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.