ZFIN ID: ZDB-FIG-131024-4
Abramsson et al., 2013 - The zebrafish amyloid precursor protein-b is required for motor neuron guidance and synapse formation. Developmental Biology   381(2):377-88 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 6 Impaired patterning of the secondary motor neurons. The Tg(isl1:GFP ) is expressed in SMNs of controls (A) but is reduced in appbMO injected embryos (B). Staining with the SMN-specific antibody zn5 shows irregular projections and stalled outgrowth of the SMN in appb morpholino injected embryos (B) compared to controls (A). The Tg(gata2:GFP) transgene shows that the irregular patterning of extending axons persist in morphants (D) while controls (C) have a regular pattern of SMN by 3 dpf. The dorsolateral fasciculus (DLF) which extends to the end of the spinal cord in controls (E) is projecting out to the tip of the tail fin in appb morphants (arrow, F). The contour of the tail fin is outlined by a dotted line. Bar: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP rw0Tg (AB) standard conditions Prim-5 secondary motor neuron IFL
rw0Tg + MO5-appb (AB) standard conditions Prim-5 secondary motor neuron IFL
zf35Tg (AB) control Protruding-mouth secondary motor neuron IFL
zf35Tg + MO5-appb (AB) standard conditions Protruding-mouth secondary motor neuron IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-5 IHC rw0Tg (AB) standard conditions Prim-5 secondary motor neuron
IHC rw0Tg + MO5-appb (AB) standard conditions Prim-5 secondary motor neuron
Phenotype Details
Fish Conditions Stage Phenotype
rw0Tg + MO5-appb (AB) standard conditions Prim-5 secondary motor neuron axon extension decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 381(2), Abramsson, A., Kettunen, P., Banote, R.K., Lott, E., Li, M., Arner, A., and Zetterberg, H., The zebrafish amyloid precursor protein-b is required for motor neuron guidance and synapse formation, 377-88, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.