FIGURE

Fig. 3

ID

ZDB-FIG-131015-10

Publication

Tappeiner et al., 2013 - Characteristics of Rod Regeneration in a Novel Zebrafish Retinal Degeneration Model Using N-Methyl-N-Nitrosourea (MNU)

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Fig. 3

TUNEL staining of zebrafish retina, 3 days after exposure to MNU 150 mg/l.

A. TUNEL-positive cells (red) are localized in the outer nuclear layer. Strong autofluorescence (green) of a cone outer segment allows identification of the corresponding nearby cone nucleus. Based on this assessment, cone photoreceptors are TUNEL-negative. B. Immunhistochemistry with zpr-1 (staining double cones, green dotted) combined with TUNEL staining (red) confirmed that cone photoreceptors are TUNEL-negative. C. Immunhistochemistry with rhodopsin (staining rods, green) and TUNEL staining (red). Co-Localization of rhodopsin and TUNEL exemplarily shows a dying rod photoreceptor (arrow). Cell nuclei are stained with DAPI (blue). INL (inner nuclear layer), RN (rod nuclei), CN (cone nuclei), CO (cone outer segment). Scale bar indicates 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	chemical treatment: pharmaceutical
Observed In:	retinal outer nuclear layer retinal rod cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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