FIGURE

Fig. S6

ID

ZDB-FIG-130828-9

Publication

Sacilotto et al., 2013 - Analysis of Dll4 regulation reveals a combinatorial role for Sox and Notch in arterial development

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Fig. S6

(A) Analysis of GFP expression in 3-dpf tg(Dll4in3:GFP) zebrafish embryos after scrambled, rbpj, double sox7;sox18, and triple sox7;sox18;rbpj MO injections. Triple sox7;sox18;rbpj MO results in total ablation of tg(Dll4in3:GFP) expression. We used 0.25 pmol for sox7 and sox18 and 0.30 pmol of rbpj MO. (B–E) Analysis of GFP and endogenous dll4 expression in tg(Dll4in3:GFP) (34 hpf) and WT zebrafish (26 hpf) after chemical inhibition of Notch signaling with 100 μM N-[N-3,5-Difluorophenacetyl]-L-alanyl-S-phenylglycine Methyl Ester (DAPM) alone (B and C) and in combination with sox7;sox18 MO (D and E). sox7; sox18 MO injection in combination with DAPM results in total ablation of both GFP and endogenous dll4 expression. (F–I) Chemical inhibition of VEGF signaling in tg(Dll4in3:GFP) zebrafish embryos with 1 μM SU5416 alone or in combination with rbpj or double sox7;sox18 MO abolishes the GFP expression. Values on the bottom right indicate number of embryos with the predominant and displayed phenotype/ total number of embryos analyzed. In all cases, 0.125 pmol of sox7 and sox18 and 0.15 pmol of rbpj MO were used.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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