FIGURE

Fig. 1

ID

ZDB-FIG-130826-50

Publication

Chapman et al., 2013 - Axonal Transport Defects in a Mitofusin 2 Loss of Function Model of Charcot-Marie-Tooth Disease in Zebrafish

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Fig. 1

A zebrafish MFN2 mutant.

(A) CLUSTAL alignment of zebrafish (Danio rerio: DR) and human (Homo sapiens: HS) Mfn2 protein sequences (Ensembl). The solid and dashed lines indicate the GTPase and transmembrane domains respectively. The site of the ENU induced point mutation (L285) is indicated with an arrowhead. (B) The ENU induced mutation creates a novel restriction enzyme site for MseI, allowing genotyping using PCR followed by MseI digest. The agarose gel shows, from left to right: Hyperladder V (Bioline), two wild type embryos (MFN2^+/+), two mutation carriers (MFN2^L285X/+), and two homozygotes (MFN2^L285X/L285X). (C) Mitochondria in cultured MFN2^L285X/L285X neurons have a significantly reduced aspect ratio (p<0.01, Kruskal-Wallis with Dunn’s multiple comparisons test). (D) Representative images of axonal mitochondria stained with Mitotracker Red in cultured neurons of the indicated genotypes.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mfn2^{hu3528/hu3528} mfn2^hu3528/+
Observed In:	neuron mitochondrion
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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