FIGURE

Fig. 1

ID

ZDB-FIG-130822-34

Publication

Sah et al., 2013 - Ion channel-kinase TRPM7 is required for maintaining cardiac automaticity

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Fig. 1

TRPM7 current in cultured murine embryonic ventricular myocytes. (A) Representative TRPM7 current measured in cultured EVM compared with AVM after full run-up. (B) Mean TRPM7 current density at +100 mV in AVM (I_Trpm7, _AVM = 8.3 ± 0.9 pA/pF, n = 5) compared with EVM (I_Trpm7, _EVM = 64.2 ± 16.7 pA/pF, n = 5). (C) Interference contrast (DIC, Left) and fluorescence GFP image (Right). (D) PCR across exon 17 from genomic DNA isolated from Trpm7^fl/fl EVM transduced with Ad-TnT-Cre (1), Ad-CMV-Cre (2), and Ad-CMV-Lacz (3), Trpm7^fl/fl fibroblasts (+), and Trpm7^fl/- tail (-). Black arrow, full-length exon 17. Red arrow, deleted exon 17. (E) Representative TRPM7 current measured in Trpm7^fl/fl EVM and Trpm7^fl/fl EVM 5 d after transduction with Ad-CMV-Cre. (F) Mean TRPM7 current density in Trpm7^fl/fl EVM treated with Ad-CMV-Cre (n = 3) compared with untreated Trpm7^fl/fl EVM (n = 5). *P < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA