Fig. 2
Zebrafish injected with rag2:KRASG12D, cdh15:KRASG12D or mylz2:KRASG12D develop muscle tumors that differ in histological appearance. (A-C) Brightfield images of fish bearing rag2 (A), cdh15 (B) and mylz2 (C) tumors. (D-G) H&E staining of tumors. (H-W) Tumors express diagnostic markers of RMS as assessed by RNA in situ hybridization. Histological sections of representative rag2 (D,H,L,P,T), cdh15 (E,I,M,Q,U) and mylz2 (F,J,N,R,V) tumors and normal muscle (G,K,O,S,W). Images in D-W are at the same magnification. (X) Tumors were assigned differentiation scores based on histological appearance. Scale bars: in A-C, 2 mm; in D-G, 50 μm. |