FIGURE

Fig. 4

ID

ZDB-FIG-130808-3

Publication

Dagliyan et al., 2013 - Rational design of a ligand-controlled protein conformational switch

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Fig. 4

Activation of Src induces cell changes in zebrafish epidermal cells. (A) Synthetic transposase mRNA was coinjected with the Tol2 Krt4 Src (YF)-uniRapR-cerulean plasmid into one-cell zebrafish embryos, resulting in mosaic expression of Src-uniRapR in the epidermis. Epidermal cells with characteristic flat honeycomb morphology were selected and imaged before (B and F) and after (C and G) 16 h of rapamycin treatment. (C) Epidermal cells expressing Src (YF)-uniRapR-cerulean in zebrafish embryos exposed to 10 μM rapamycin become rounded and undergo dynamic cell shape changes. White arrows indicate an epidermal cell before and after each treatment. Control epidermal cells expressing Src (YF)-uniRapR-cerulean in vehicle (i.e., DMSO) (D and E) or expressing the kinase-dead construct Src (YF/KD)-uniRapR-cerulean in rapamycin (G) have a static morphology and do not undergo dynamic cell shape changes. (Scale bar: 30 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA