Fig. 4

Recovery and adaptation of neuronal activity in 300

mM ethanol. Fluorescence intensities of Mauthner neurons and MiD3 neurons were measured in response to sound/vibration stimuli. Sectioned larvae were either washed after 30 min incubation of 300 mM ethanol (A) or bathed continuously in 300 mM ethanol (D, E). Black, red, green and blue traces correspond to 0, 30, 60 and 90 min, respectively. The peaks of ΔF/F normalized to 0 min were plotted against time in B (for A) and F (for D, E) (*P<0.05, ***P<0.001 versus 0 min). Five Mauthner neurons and six MiD3 neurons were measured. Stimuli were applied and calcium transients were elicited at each concentration 2–5 times. The numbers of samplings are shown in parentheses. (C) AO staining in 0 mM (top) and 300 mM (bottom) ethanol are shown. Apoptosis in olfactory organs is marked with arrowheads. Apoptosis of neuromasts, located on the body surface, are shown with arrowheads in insets. Nonspecific signals are observed in the gut (Arrows).