FIGURE

Fig. 5

ID

ZDB-FIG-130619-18

Publication

Ren et al., 2013 - Activated N-Ras signaling regulates arterial-venous specification in zebrafish

Other Figures

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Fig. 5

Vasculogenesis is disrupted in hNRASD12-expressing transgenic embryos. (A-B) Confocal images of trunk vasculature of living control; flk1:GFP (A) and hNRASD12;flk1:GFP embryos (B) at 28 hpf. DA, dorsal aorta; PCV, posterior cardinal vein; ISV, intersegmental vessel. (C-D) Mid-trunk transverse sections of control;flk1:GFP (C) and hNRASD12;flk1:GFP embryos (D) at 28 hpf stained by GFP antibody (green) and Phalloidin-TRITC (red). NT, neural tube; NC, notochord. (E-J) Confocal images of head vasculature of living control;flk1:GFP (E, G, I) and hNRASD12;flk1:GFP embryos at 28 hpf (F, H, J). LDA, lateral dorsal aorta; PHBC, primordial hindbrain channel; CCV, common cardinal vein; BA, basilar artery. The red dotted lines denote the boundaries of the CCVs and PCVs respectively.

Expression Data

Gene:	EGFP
Fish:	cas001Tg; rj5Tg; s843Tg cas001Tg; s843Tg
Anatomical Terms:	basilar artery common cardinal vein cranial vasculature dorsal aorta intersegmental vessel lateral dorsal aorta posterior cardinal vein primordial hindbrain channel trunk vasculature (all 9)
Stage:	Prim-5

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
EGFP	cas001Tg; rj5Tg; s843Tg	standard conditions	Prim-5	cranial vasculature	IFL
EGFP	cas001Tg; rj5Tg; s843Tg	standard conditions	Prim-5	trunk vasculature	IFL
EGFP	cas001Tg; rj5Tg; s843Tg	standard conditions	Prim-5	trunk vasculature	IHC
EGFP	cas001Tg; s843Tg	standard conditions	Prim-5	basilar artery	IFL
EGFP	cas001Tg; s843Tg	standard conditions	Prim-5	common cardinal vein	IFL
EGFP	cas001Tg; s843Tg	standard conditions	Prim-5	cranial vasculature	IFL
EGFP	cas001Tg; s843Tg	standard conditions	Prim-5	dorsal aorta	IHC
EGFP	cas001Tg; s843Tg	standard conditions	Prim-5	dorsal aorta	IFL
EGFP	cas001Tg; s843Tg	standard conditions	Prim-5	intersegmental vessel	IHC
EGFP	cas001Tg; s843Tg	standard conditions	Prim-5	intersegmental vessel	IFL

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Antibody Labeling

Phenotype Data

Fish:	cas001Tg; rj5Tg; s843Tg
Observed In:	basilar artery common cardinal vein cranial vasculature head blood circulation lateral dorsal aorta posterior cardinal vein trunk vasculature (all 7)
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Hematol. Oncol.