Fig. 2

Models of coordinate and independent DV and AP patterning, and anteriorization by FGF inhibition is patterned by BMP signaling with the same temporal dynamics as the normal domain. (A) Models in which DV and AP patterning are coordinated (model 1) or independent (model 2). Embryos are represented at late gastrula stage. The red dashed lines indicate the posteriorly expanded anterior tissue in the top panels and the anteriorly expanded posterior tissue in the bottom panels. (B-K) otx2 expression in anterior neurectoderm in non-heat shocked Tg(hsp70:chd) zebrafish embryos (WT) (B,G) and following HS at the indicated stages (C-F,H-K), with (G-K) or without (B-F) inhibition of FGF signaling by SU5402. The DV width and the AP length of otx2 expression are indicated by horizontal and vertical arrows, respectively. Lateral views, dorsal to right, at 80% epiboly. B, n=30/30; C, n=27/31; D, n=25/29; E, n=21/21; F, n=37/39; G, n=37/39; H, n=34/37; I, n=37/40; J, n=23/25; K, n=34/35.