Fig. 4
- ID
- ZDB-FIG-130528-11
- Publication
- Flasse et al., 2013 - The bHLH transcription factor Ascl1a is essential for the specification of the intestinal secretory cells and mediates Notch signaling in the zebrafish intestine
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All the cells of the gut epithelium adopt either a goblet or enteroendocrine fate in the mib mutant. Ventral views with anterior to the left of wt or mib embryos labeled by immunohistochemistry with 2F11 together with the binding of fluorescent WGA (A and B) or by WISH using the agr2 probe (C and D) or the fabp2 probe (E and F). A and B: The gut of 5-day mib mutants is nearly completely covered by secretory cells, either enteroendocrine cells (2F11+/WGA?), found preferentially in the intestinal bulb and the midgut, or goblet cells (2F11+/WGA+). C and D: The presence of goblet cells in the gut of mib mutant at 4 dpf was confirmed by WISH using the agr2 probe. The double-headed arrows indicate the length of the gut. E and F: 84 hpf mib larvae display a nearly complete loss of fabp2-labeled enterocytes. Scale bars: 50 μM. |
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| Stage Range: | Protruding-mouth to Day 5 |
Reprinted from Developmental Biology, 376(2), Flasse, L., Stern, D.G., Pirson, J.L., Manfroid, I., Peers, B., and Voz, M.L., The bHLH transcription factor Ascl1a is essential for the specification of the intestinal secretory cells and mediates Notch signaling in the zebrafish intestine, 187-197, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.