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ZFIN ID: ZDB-FIG-130524-17
Chou et al., 2013 - Fibronectin mediates correct positioning of the interrenal organ in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   242(5):432-443 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 4 The interaction between interrenal and chromaffin cells in the fn1 mutant at 34 and 54 hpf. Double ISH assays showing the colocalization of ff1b (red) with dbh (black) in the class I and II fn1 mutants, as well as their wild-type siblings. Ventral flat mount (A–A′′, C–C′′, E–E′′, G–G′′, I–I′′, K–K′′) and lateral (B–B′′, D–D′′, F–F′′, H–H′′, J–J′′, L–L″) views are shown for the representative embryo of each phenotypic type (n =17, 10, and 24 for the 34-hpf fn1 mutant class I, class II, and their wild-type siblings, respectively; n = 6, 3, and 18 for the 54-hpf fn1 mutant class I, class II, and their wild-type siblings, respectively). The anterior (A) versus posterior (P) orientation of each sample is indicated, and the midline is marked by yellow dotted lines. The embryos in the lateral view panels are oriented with the dorsal side to the top, and migrating chromaffin cell clusters are highlighted by white arrowheads. The initial interaction between interrenal and chromaffin cell populations appears normal; while the continuous recruitment of chromaffin cells to the interrenal region is defective in the fn1 mutant. Scale bar = 50 μM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbh WT standard conditions Prim-15 chromaffin cell ISH
Long-pec chromaffin cell ISH
fn1akt259/kt259 standard conditions Prim-15 chromaffin cell ISH
Long-pec chromaffin cell ISH
nr5a1a WT standard conditions Prim-15 interrenal primordium ISH
Long-pec interrenal primordium ISH
fn1akt259/kt259 standard conditions Prim-15 interrenal primordium ISH
Long-pec interrenal primordium ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-acta2 IHC WT standard conditions Prim-15 chromaffin cell
IHC Long-pec chromaffin cell
Phenotype Details
Fish Conditions Stage Phenotype
fn1akt259/kt259 standard conditions Prim-15 chromaffin cell located in interrenal primordium, normal
Prim-15 interrenal primordium left side unfused from interrenal primordium right side, abnormal
Long-pec chromaffin cell located in interrenal gland, normal
Long-pec interrenal gland mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.