Fig. 4
- ID
- ZDB-FIG-130509-25
- Publication
- Yang et al., 2013 - A novel zebrafish xenotransplantation model for study of glioma stem cell invasion
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CD133 expression on GSCs is reduced after spread in zebrafish embryos. A. CD133 expression examined on non-invasive or invasive cells in the embryos injected with CD133+ U87 GSCs. All CD133+ U87 GSCs within injected embryos were stained with a monoclonal anti-CD133 antibody (1:300) and examined by confocal microscopy. Green: EGFP-labeled endothelial cells; red: RFP-labeled U87 sphere cells; blue: CD133+ U87 GSCs. B. The percentage of CD133+ cells in non-invasive cells or invasive cells at 2 dpi (n = 300 each group). C. Detection of CD133+ cell percentage in sorted CD133+ U87 cells in preparation for microinjection by flow cytometry (p<0.001). |