ZFIN Image: Yang et al., 2013, Fig. 4

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Figure Caption

Fig. 4 CD133 expression on GSCs is reduced after spread in zebrafish embryos.

A. CD133 expression examined on non-invasive or invasive cells in the embryos injected with CD133⁺ U87 GSCs. All CD133⁺ U87 GSCs within injected embryos were stained with a monoclonal anti-CD133 antibody (1:300) and examined by confocal microscopy. Green: EGFP-labeled endothelial cells; red: RFP-labeled U87 sphere cells; blue: CD133⁺ U87 GSCs. B. The percentage of CD133⁺ cells in non-invasive cells or invasive cells at 2 dpi (n = 300 each group). C. Detection of CD133⁺ cell percentage in sorted CD133⁺ U87 cells in preparation for microinjection by flow cytometry (p<0.001).

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