FIGURE

Fig. 1

ID
ZDB-FIG-130416-20
Publication
Navis et al., 2013 - Cftr controls lumen expansion and function of Kupffer's vesicle in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

Generation of a cftr mutant zebrafish. (A) A TALEN was designed to target the EcoRV site in the sixth transmembrane domain. The spacer is marked by green text and the restriction site is denoted by red text. (B) Schematic of the domain structure of Cftr with a star indicating the TALEN target site within the third transmembrane domain of the protein. NBD, nucleotide binding domain; R, regulatory domain. (C) TALEN activity generated insertions and deletions that disrupt the EcoRV site. (D) Sequence alignments of the TALEN-generated alleles. Cftrpd1048 has a six-nucleotide deletion, cftrpd1049 and cftrpd1050 have nucleotide insertions and deletions causing frameshifts leading to premature stop codons. (E) An alignment of the amino acid sequences encoded by cftrpd1049 and WT cftr.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development