Fig. 1

Fig. 1 Generation of a cftr mutant zebrafish. (A) A TALEN was designed to target the EcoRV site in the sixth transmembrane domain. The spacer is marked by green text and the restriction site is denoted by red text. (B) Schematic of the domain structure of Cftr with a star indicating the TALEN target site within the third transmembrane domain of the protein. NBD, nucleotide binding domain; R, regulatory domain. (C) TALEN activity generated insertions and deletions that disrupt the EcoRV site. (D) Sequence alignments of the TALEN-generated alleles. Cftr^pd1048 has a six-nucleotide deletion, cftr^pd1049 and cftr^pd1050 have nucleotide insertions and deletions causing frameshifts leading to premature stop codons. (E) An alignment of the amino acid sequences encoded by cftr^pd1049 and WT cftr.