FIGURE

Fig. 1

ID

ZDB-FIG-130404-22

Publication

Doganli et al., 2012 - The alpha2Na+/K+-ATPase is critical for skeletal and heart muscle function in zebrafish

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Fig. 1

Expression of Atp1a2 mRNA in zebrafish embryos. (A) Atp1a2 mRNA expression was quantified by qRT-PCR and normalized to Actb2. Data are presented as means ± s.e.m. of triplicate measurements. (B) Atp1a2 mRNA expression analyzed by whole-mount in situ hybridization in zebrafish embryos at 35 hpf (NBT/BCIP stained). The inset is a dorsal view of caudal staining at 35 hpf, and 60 hpf (Fast Red staining). Brackets indicate the position of the double-stained sections in C. Lower panel show sense probe negative controls. (C) Transverse sections of embryos double stained by in situ hybridization for Atp1a2 (red fluorescence) in combination with immunostaining using F310 (fast-twitch muscle) or F59 (primarily slow-twitch muscle; green fluorescence). H, heart; M, myotomes; N, notochord; OB, olfactory bulb; PF, pectoral fin; SC, spinal cord; T, midbrain tegmentum; VT, ventral thalamus. Scale bars: 100 μm.

Expression Data

Gene:	atp1a2a
Antibodies:	Ab-F59 Ab-F310
Fish:	TU
Anatomical Terms:	brain fast muscle cell heart olfactory bulb pectoral fin skeletal muscle slow muscle cell tegmentum ventral thalamus whole organism
Stage Range:	1k-cell to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.