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ZFIN ID: ZDB-FIG-130326-14
Lenhart et al., 2013 - Integration of Nodal and BMP Signals in the Heart Requires FoxH1 to Create Left-Right Differences in Cell Migration Rates That Direct Cardiac Asymmetry. PLoS Genetics   9(1):e1003109 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage Range: 20-25 somites to 26+ somites
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: 20-25 somites to 26+ somites

Fig. 1 Trajectories and average velocities of migrating cells within the cardiac cone.

A–G: First frame of time lapse movies taken from a dorsal view of Tg(myl7:eGFP) embryos overlaid with cell trajectories. Yellow tracks: left cells. White tracks: center cells. Red tracks: right cells. A: All cells exhibit trajectories directed toward the anterior and left in WT embryos. B: Bilateral expression of spaw in ntl morphants leads to loss of L/R asymmetry in cardiac cell migrations. C–G: L/R directionality is also absent in embryos C: lacking the Nodal ligand spaw; D: treated with the SB-505124 Nodal inhibitor drug E: lacking spaw and one functional copy of bmp4; F: injected with both bmp4 and spaw morpholinos; and G: homozygous midway mutants. H: Numbers within each bar indicate the number of cells tracked on the left, center and right for all embryos of each genotype. The number of embryos utilized in analysis is indicated (n = ) for each genotype. WT embryos exhibit differences in average cell velocities along the L/R axis, with left cells migrating significantly faster than right cells. Bilateral exposure to Spaw leads to significant increases in average velocities in left and right cells of ntl morphants, while loss of Spaw and global inhibition of Nodal signaling through drug treatment results in significantly decreased rates of migration. In embryos with diminished Bmp signaling along with loss of Spaw, average cell velocities are significantly increased compared with loss of Spaw alone. Error bars in H indicate standard error of the mean. MO: morpholino. Het: Heterozygotes.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP bmp4b1172/+; twu34Tg + MO3-spaw standard conditions 20-25 somites to 26+ somites heart rudiment IFL
foxh1pr1/pr1; twu34Tg standard conditions 20-25 somites to 26+ somites heart rudiment IFL
twu34Tg standard conditions 20-25 somites to 26+ somites heart rudiment IFL
twu34Tg chemical treatment: SB 505124 20-25 somites to 26+ somites heart rudiment IFL
twu34Tg + MO1-tbxta standard conditions 20-25 somites to 26+ somites heart rudiment IFL
twu34Tg + MO3-bmp4 + MO3-spaw standard conditions 20-25 somites to 26+ somites heart rudiment IFL
twu34Tg + MO3-spaw standard conditions 20-25 somites to 26+ somites heart rudiment IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bmp4b1172/+; twu34Tg + MO3-spaw standard conditions 20-25 somites - 26+ somites embryonic heart tube left/right pattern formation process quality, abnormal
20-25 somites - 26+ somites heart rudiment cardioblast anterior-lateral migration process quality, abnormal
foxh1pr1/pr1; twu34Tg standard conditions 20-25 somites - 26+ somites embryonic heart tube left/right pattern formation process quality, abnormal
20-25 somites - 26+ somites heart rudiment cardioblast anterior-lateral migration process quality, abnormal
twu34Tg chemical treatment: SB 505124 20-25 somites - 26+ somites embryonic heart tube left/right pattern formation process quality, abnormal
twu34Tg chemical treatment: SB 505124 20-25 somites - 26+ somites heart rudiment cardioblast anterior-lateral migration process quality, abnormal
twu34Tg + MO1-tbxta standard conditions 20-25 somites - 26+ somites embryonic heart tube left/right pattern formation process quality, abnormal
20-25 somites - 26+ somites heart rudiment cardioblast anterior-lateral migration process quality, abnormal
twu34Tg + MO3-bmp4 + MO3-spaw standard conditions 20-25 somites - 26+ somites embryonic heart tube left/right pattern formation process quality, abnormal
20-25 somites - 26+ somites heart rudiment cardioblast anterior-lateral migration process quality, abnormal
twu34Tg + MO3-spaw standard conditions 20-25 somites - 26+ somites embryonic heart tube left/right pattern formation process quality, abnormal
20-25 somites - 26+ somites heart rudiment cardioblast anterior-lateral migration process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.