FIGURE

Fig. 3

ID
ZDB-FIG-130318-30
Publication
Qian et al., 2013 - ENC1-like Integrates the Retinoic Acid/FGF Signaling Pathways to Modulate Ciliogenesis of Kupffer's Vesicle during Zebrafish Embryonic Development
Other Figures
All Figure Page
Back to All Figure Page
Fig. 3

Enc1l is essential for KV ciliogenesis and KV function. (A–F) Anti-acetylated tubulin antibody is used to visualize the cilia in KV. (A) Control embryos, (B) enc1l-MO1 is injected at 1-cell stage, (C) enc1l-MO1 and enc1l-mRNA are co-injected at 1-cell stage. (D) enc1l-MO1 is injected at the 1000-cell stage (EMO1-1K). (E, F) Statistical graphs show the decrease in cilium length and increase in cilium number. (G–L): Enc1l is required for counterclockwise nodal fluid flow. (G–I) Still images from control-MO embryos and (J–L) enc1l-MO injected embryos. These images are taken from supplement Movie1 and 2, respectively. (G, J): Fluorescent beads in the KV. (H, K): The KV structure from the DIC image. (I, L): Trajectory of the fluorescent beads in KV. **0.005<π<0.01; *** :p<0.005.

Expression Data
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: 1-4 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: 1-4 somites

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 374(1), Qian, M., Yao, S., Jing, L., He, J., Xiao, C., Zhang, T., Meng, W., Zhu, H., Xu, H., and Mo, X., ENC1-like Integrates the Retinoic Acid/FGF Signaling Pathways to Modulate Ciliogenesis of Kupffer's Vesicle during Zebrafish Embryonic Development, 85-95, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.