ZFIN ID: ZDB-FIG-130228-35
Parente et al., 2013 - Hypoxia/Reoxygenation cardiac injury and regeneration in zebrafish adult heart. PLoS One   8(1):e53748 Full text @ PLoS One
Anatomical Terms:
Stage: Adult

Fig. 2 Inflammatory response induced by H/R in vivo.

Representative confocal microscopy images (a-h) showing neutrophils (yellow fluorescence or green fluorescence) and macrophages (red fluorescence) infiltration in double transgenic line Tg(MPO:EGFP)×Tg(LysC:DsRed) in control (C) and at different time points (4 h, 6 h, and 14 h) after H/R. Neutrophils are either yellow (LysC+/MPO+) or green (predominantly, MPO+) cells (arrows in the 6 h image); red macrophages are LysC+ cells (arrow in the 4 h image). Hoechst stains cell nuclei; (a-d) calibration bar = 100 µm, (e-h) calibration bar = 20 µm.The peak inflammatory response occurred at the 6 h time point after H/R This experiment was performed three times with similar results.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 i114Tg; nz50Tg hypoxia Adult macrophage IHC
Adult neutrophil IHC
GFP i114Tg; nz50Tg hypoxia Adult neutrophil IHC
Antibody Labeling Details No data available
Phenotype Details No data available
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