FIGURE

Fig. S1

ID

ZDB-FIG-121212-3

Publication

Herbert et al., 2012 - Determination of Endothelial Stalk versus Tip Cell Potential during Angiogenesis by H2.0-like Homeobox-1

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Fig. S1

Pharmacological Manipulation of EC Sprouting and hlx1 Expression, Related to Figure 1
(A-B) Lateral views of Tg(kdrl:nlsEGFP)^zf109 embryos upon incubation with either DMSO or the indicated concentration of SU5416 from 22 to 30 hpf (A) and quantification of ISV EC numbers (B). SU5416 dose-dependently disrupted EC sprouting behaviour.
(C) Fold expression change of hlx1 and known EC genes in expression profiles of ECs upon incubation with 2.5 μM SU5416 or 100 μM DAPT versus DMSO control. Expression of hlx1 is highly SU5416 and DAPT responsive relative to other known EC genes.
(D) Correlation between the total number of sprouting ECs observed following chemical manipulation of ISV angiogenesis (taken from Figures 1C and D) versus the fold change in EC expression of flt4 and hlx1 (taken from Figure 1F). flt4 and hlx1 expression levels were highly correlated with EC sprouting. Error bars represent mean ± SEM. (*P < 0.05 versus DMSO).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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