FIGURE

Fig. S3

ID

ZDB-FIG-121212-17

Publication

Dumortier et al., 2012 - Collective mesendoderm migration relies on an intrinsic directionality signal transmitted through cell contacts

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Fig. S3

Role of RhoA and Cdc42 on cytoplasmic extensions orientation. Cells injected with RNAs encoding Lifeact-mCherry and DN-RhoA (A and B) or DN-Cdc42 (D and E) were transplanted from shield to shield, in Tg(gsc:GFP) embryos. Transplanted cells are within the prechordal plate (assessed by GFP expression), and contour of the plate is delineated (white line). Orientations of their cytoplasmic extensions were measured relative to the animal pole and plotted as histograms (B and E) or cumulative plot (F). Frequencies of cytoplasmic extensions are plotted in C. No difference could be observed between control cells and cells expressing DN-Cdc42 (P = 0.84, Kolmogorov?Smirnov test; n = 537 and n = 269 extensions). Expression of DN-RhoA affected the distribution of extension orientations (P = 6.105, Kolmogorov?Smirnov test; n = 537 and n = 524 extensions), even though it did not affect the average orientation (P = 0.35, Student t test). Animal pole is to the left in A and D.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA