Fig. 3
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- ZDB-FIG-121210-18
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- Cao et al., 2012 - High mobility group B proteins regulate mesoderm formation and dorsoventral patterning during zebrafish and Xenopus early development
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Effects of different hmgb3 mutants on mesoderm gene expression and on early development in zebrafish. Embryos were injected with different mRNAs corresponding to different hmgb3 mutants as indicated on the left and in situ hybridization was performed using the markers indicated on the top. (A-D) Dorsal view (expect B, which is animal pole view) of gsc expression at 50% epiboly. (A) A control embryo. (B) A hmgb3EnR-injected embryo shows expansion of gsc expression. (C) A hmgb3VP16-injected embryo shows weakly inhibited gsc expression. (D) A hmgb3ΔC-injected embryo with expansion of gsc expression. (E–H) Animal pole view of ntl expression at 50% epiboly. The uninjected control embryo (E) shows circular ntl expression at the margin, while the hmgb3EnR-injected embryo (F) shows strong inhibition of ntl expression. hmgb3VP16 (G) has no obvious effect on ntl expression and hmgb3ΔC (H) moderately inhibits ntl expression. (I-L) Animal pole view of eve1 expression at 50% epiboly. (I) An uninjected embryo. (J) hmgb3EnR blocks eve1 expression. (K) hmgb3VP16 does not affect eve1 expression pattern. (L) hmgb3ΔC inhibits eve1 expression to a lesser extent than hmgb3EnR. (M–P) Lateral view of live images of control (M) and injected embryos at 48 hpf with anterior to the left. The embryos injected with hmgb3EnR (N) or hmgb3ΔC (P) mRNA show trunk and posterior deficiency, while the hmgb3VP16-injected embryo (O) shows weak anterior deficiency. (Q) Summary of the phenotypes following overexpression of different hmgb mRNAs and hmgb3 mutants. Severely affected embryos are as shown in N with absence of trunk and posterior regions, mild phenotypes are intermediate between normal and severely affected, as shown in Fig. 1N-P. Numbers on the top indicate total embryos scored from four independent experiments. (R) Summary of the phenotypes following hmgb3VP16 overexpression. Severely affected embryos show absence of eyes, while mild phenotypes are as shown in O. Numbers on the top indicate total embryos scored from three independent experiments. |
Reprinted from Mechanisms of Development, 129(9-12), Cao, J.M., Li, S.Q., Zhang, H.W., and Shi, D.L., High mobility group B proteins regulate mesoderm formation and dorsoventral patterning during zebrafish and Xenopus early development, 263-274, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.