Fig. 6

Disruption of Celf1-dmrt2a mRNA interaction cancels the effects of celf1 overexpression on somite symmetry and LR asymmetric patterning. (A-D) Representative images of uncx4.1 expression showing left-biased (celf1 mRNA plus control-MO; A), right-biased (celf1 mRNA plus control-MO; B) or symmetric (celf1 mRNA plus dmrt2a-TP-MO; C,D) somitogenesis in zebrafish embryos at 12-14 hpf. Dorsal view, anterior to the top. Asterisks mark the last-formed somite. (E-H) Representative images of mlc2a expression showing R-jog (celf1 mRNA plus control-MO; E), no-jog (celf1 mRNA plus control-MO; F) or L-jog (celf1 mRNA plus dmrt2a-TP-MO; G,H) of the heart in embryos at 24-28 hpf. Dorsal view, anterior to the top. Arrows indicate the direction of heart jogging. (I) Percentages of symmetric (L=R), left-biased (L>R) or right-biased (L<R) asymmetric somitogenesis in embryos injected with 50 pg celf1 mRNA and either 5 ng control-MO (n=70), 2.5 ng dmrt2a-TP-MO (n=43), 5 ng dmrt2a-TP-MO (n=79), 2.5 pg mRFP mRNA (n=55), 1.25 pg dmrt2a mRNA (n=69) or 2.5 pg dmrt2a mRNA (n=70) which lacks Celf1-binding site. (J) Percentages of L-jog, R-jog, no-jog, or cardia bifida of the heart in embryos injected with 50 pg celf1 mRNA and either 5 ng control-MO (n=98), 2.5 ng dmrt2a-TP-MO (n=62), 5 ng dmrt2a-TP-MO (n=92), 2.5 pg mRFP mRNA (n=56), 1.25 pg dmrt2a mRNA (n=55) or 2.5 pg dmrt2a mRNA (n=62), which lacks Celf1-binding site. Scale bars: 200 μm.