FIGURE

Fig. 2

ID

ZDB-FIG-121030-41

Publication

Shwartz et al., 2012 - Muscle contraction controls skeletal morphogenesis through regulation of chondrocyte convergent extension

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Fig. 2

Neural crest specification, migration and differentiation in the absence of muscle contraction. (A) sox10 expression (green) in confocal images of control (upper panel) and paralyzed (lower panel) 25 hpf zebrafish embryos; numbers indicate pharyngeal arches. (B) In situ hybridization of control (upper panels) and nic mutant (lower panels) embryos demonstrates the expression patterns of sox9a (left), col2a1 (middle) and barx1 (right) at 48 hpf (a) and at 72 hpf (b). Green arrows indicate ceratobranchial cartilage, and black arrows indicate Meckel′s cartilage and ceratohyal cartilage. (C) Immunostaining for col2a1 (red) of control (upper panel) and nic mutant 120 hpf zebrafish (lower panel). (D) Counting of cells that compose both ceratohyal cartilages of five controls and six 120 hpf nic mutants (p=0.4909). Scale bars are 100 μm.

Expression Data

Genes:	barx1 col2a1a sox9a
Fish:	WT chrna1^b107/b107
Anatomical Terms:	ceratobranchial cartilage ceratohyal cartilage Meckel's cartilage palatoquadrate cartilage
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	chrna1^b107/b107
Observed In:	ceratohyal cartilage chondrocyte pharyngeal arch cartilage
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 370(1), Shwartz, Y., Farkas, Z., Stern, T., Aszódi, A., and Zelzer, E., Muscle contraction controls skeletal morphogenesis through regulation of chondrocyte convergent extension, 154-163, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.