FIGURE

Fig. 7

ID

ZDB-FIG-121025-46

Publication

Ben Shoham et al., 2012 - S1P1 inhibits sprouting angiogenesis during vascular development

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Fig. 7

Excessive filopodia formation leads to aberrant vascular remodeling in s1p₁ MO zebrafish and S1P₁^-/- mouse embryos. (A) Sequential confocal microscopy images showing lateral views of the CVPs from WT and s1p₁ MO Tg(fli-egfp)^y1 zebrafish embryos. Circles demarcate two sprouts, marked as 1 and 2, in WT and s1p₁ MO embryos that extend filopodia (marked with asterisks). This results in the formation of a capillary loop in the WT and in over-sprouting and fusion in the mutant. For visualization of the entire sequence, see supplementary material Movies 1 and 2. (B) Immunofluorescent staining with anti-CD31 of whole-mount forelimbs from E9.5 WT (a,b) and S1P₁^-/- (c,d) mice; b and d show magnifications of a and c, respectively. Asterisks indicate excessive filopodia in S1P₁^-/- limbs, compared with WT limbs. Scale bars: in a,c, 50 μm; in b,d, 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO2-s1pr1
Knockdown Reagent:	MO2-s1pr1
Observed In:	caudal vein plexus endothelial cell endothelial cell filopodium filopodium assembly regulation of blood vessel remodeling sprouting angiogenesis
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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