FIGURE

Fig. 5

ID

ZDB-FIG-121025-29

Publication

Huang et al., 2012 - High-throughput screening for bioactive molecules using primary cell culture of transgenic zebrafish embryos

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Fig. 5

Test of Candidate Compounds in Mammalian Cells (A) Candidate compounds enhanced HUVEC proliferation significantly at 1 μM or 10 μM (p = 0.0007, 0.005, 9.20E-05, 0.001, 1.40E-06, 0.021, 0.009, 2.70E-05, 0.02, 0.02,1.00E-6, and 2.0E-04 for SB431542, A65, A79, A69, A86, A54, A36, Gh, Nd, Dip, Fh, and SST, respectively) compared with 1% DMSO control. Data from four independent experiments were analyzed. (B–E) Candidate compounds enhanced HUVEC migration. HUVEC migration was analyzed 24 hr after scratch wounds and drugs were added (B, C, D, and E at 0 hr; B2, C2, D2, and E2 at 24 hr after treatment). Migration distances were measured in micrometers and marked with white arrowheads. (F) Akt phosphorylation levels increased in HUVECs by candidate compounds. Akt phosphorylation assay was performed using HUVECs treated with candidate small molecules for 8 hr (A65, A86, Dip, and Fh) and positive compound (SB) compared with DMSO control. (G) Western blot band intensity was analyzed using the ImageJ program. After normalization against total Akt levels and β-actin levels, the compounds enhanced Akt phosphorylation levels by 1.6- to 6.5-fold compared with DMSO control. (H) Candidate compounds promoted Flk1 expression in differentiating mESCs. Cells were analyzed on day 4 of differentiation by flow cytometry for percentage of Flk1+. Expression levels were normalized to levels with DMSO. See also Figure S6.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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